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Int J Food Microbiol ; 264: 53-62, 2018 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-29111498

RESUMO

Yeasts play a crucial role in cheese ripening. They contribute to the curd deacidification, the establishment of acid-sensitive bacterial communities, and flavour compounds production via proteolysis and catabolism of amino acids (AA). Negative yeast-yeast interaction was observed between the yeast Yarrowia lipolytica 1E07 (YL1E07) and the yeast Debaryomyces hansenii 1L25 (DH1L25) in a model cheese but need elucidation. YL1E07 and DH1L25 were cultivated in mono and co-cultures in a liquid synthetic medium (SM) mimicking the cheese environment and the growth inhibition of DH1L25 in the presence of YL1E07 was reproduced. We carried out microbiological, biochemical (lactose, lactate, AA consumption and ammonia production) and transcriptomic analyses by microarray technology to highlight the interaction mechanisms. We showed that the DH1L25 growth inhibition in the presence of YL1E07 was neither due to the ammonia production nor to the nutritional competition for the medium carbon sources between the two yeasts. The transcriptomic study was the key toward the comprehension of yeast-yeast interaction, and revealed that the inhibition of DH1L25 in co-culture is due to a decrease of the mitochondrial respiratory chain functioning.


Assuntos
Queijo/microbiologia , Debaryomyces/crescimento & desenvolvimento , Debaryomyces/metabolismo , Perfilação da Expressão Gênica/métodos , Yarrowia/crescimento & desenvolvimento , Yarrowia/metabolismo , Aminoácidos/metabolismo , Amônia/metabolismo , Técnicas de Cocultura , Debaryomyces/genética , Aromatizantes/análise , Microbiologia de Alimentos , Ácido Láctico/metabolismo , Lactose/metabolismo , Interações Microbianas/genética , Interações Microbianas/fisiologia , Transcriptoma/genética
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